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hrp conjugated goat anti rabbit igg secondary antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno hrp conjugated goat anti rabbit igg secondary antibody
    Hrp Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 7244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hrp/Peroxidase+AffiniPure+Goat+Anti-Rabbit+IgG/pm42921007-125-25-35
    Average 96 stars, based on 7244 article reviews
    hrp conjugated goat anti rabbit igg secondary antibody - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Purification:

    Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration
    Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an HRP-coupled secondary antibody that was purchased from Jackson Immuno Research (Cat. #: 711-036-152; 1:500) in conjunction with the immunohistochemistry staining kit ImmPACT VIP Kit (Vector Laboratories, Cat# SK-4605) as shown in ( ). .. Fluorescent sections were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat# 9542) to visualize nuclei.

    Immunohistochemistry:

    Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration
    Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an HRP-coupled secondary antibody that was purchased from Jackson Immuno Research (Cat. #: 711-036-152; 1:500) in conjunction with the immunohistochemistry staining kit ImmPACT VIP Kit (Vector Laboratories, Cat# SK-4605) as shown in ( ). .. Fluorescent sections were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat# 9542) to visualize nuclei.

    Staining:

    Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration
    Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an HRP-coupled secondary antibody that was purchased from Jackson Immuno Research (Cat. #: 711-036-152; 1:500) in conjunction with the immunohistochemistry staining kit ImmPACT VIP Kit (Vector Laboratories, Cat# SK-4605) as shown in ( ). .. Fluorescent sections were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, Cat# 9542) to visualize nuclei.

    Western Blot:

    Article Title: TONSL/TONSOKU binding to histone H3.1 is required for DNA repair
    Article Snippet: The following primary antibodies were used for immunoblotting: anti-TONSL (NFKBIL2; MA5-17292, Thermo Fisher Scientific) and anti–α-tubulin (CP06, MilliporeSigma). .. Secondary antibodies used for immunoblotting were Goat anti-Rabbit immunoglobulin G (IgG), HRP (111-035-144, Jackson ImmunoResearch Laboratories Inc.), and rabbit anti-mouse-HRP (A9044, MilliporeSigma). .. Primary antibodies used for immunofluorescence were anti–phospho-histone H2A.X (Ser 139 ; 2577L, Cell Signaling Technology) and anti-FLAG (637301, BioLegend).

    other:

    Article Title: Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development
    Article Snippet: Membranes blocked in 5% nonfat milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature, followed by overnight incubation at 4°C with primary antibodies, including anti-HA (1:5000, Abcam, catalog no. Ab18181), anti-GFP (1:5000, Invitrogen, catalog no. 33-2600), anti-RFP (1:2000, ChromoTek, catalog no. pabr1), anti-FLAG (1:5000, Sigma-Aldrich, catalog no. F3165), and anti-ACTIN (1:10,000, Abcam, catalog no. Ab230169 ).

    Incubation:

    Article Title: Modifying VEGF-A mRNA by combinatorial optimization to enhance therapeutic efficacy for myocardial infarction.
    Article Snippet: After blocking, membranes were incubated overnight at 4°C with primary antibodies: rabbit anti-FLAG (1:1000, Cell Signaling Technology, #14793) and mouse antiGAPDH (1:5000, Proteintech, 60004-1-Ig). .. Membranes were washed five times with PBST (10 min each) and incubated with HRP-conjugated secondary antibodies (goat anti-rabbit IgG, 1:5000, Jackson ImmunoResearch; goat antimouse IgG, 1:5000, Jackson ImmunoResearch) for 1 h at room temperature in AR TIC LE IN PR ES S the dark. .. Following five additional PBST washes, chemiluminescent signals were detected using ECL substrate (Thermo Fisher) on a ChemiDoc imaging system (Bio-Rad).

    Article Title: DEAD-box RNA helicase DDX3X maintains the homeostasis of the Zika virus translation-replication cycle
    Article Snippet: The antibodies used were anti-β-actin (sc-477778, mouse, 1:5000, Santa Cruz Biotechnologies), anti-DDX3X (sc-365768, mouse, 1:3000, Abcam), anti-RIG-I (20566-1-AP, rabbit, 1:1000, Proteintech), DENV-2 anti-NS3 (Cat. 124252, rabbit, 1:3000, Genetex), ZIKV anti-NS3 and ZIKV anti-NS5 (rabbit, 1:3000, gently donated by Dr. Andres Merits, University of Tartu, Estonia). .. After incubation, the membranes were washed 3 times with TBS-T 1X and then incubated for 2 hours at room temperature with HRP-conjugated secondary antibody (Cat. 111-035-003, 1:5000, Jackson Immunoresearch). .. Finally, for the detection and digitalization of images, the membrane was incubated with the chemiluminescent solution for Western blot EZ-ECL (ThermoFisher Scientific) and registered with a Q9 Alliance UVITEC-Cambridge digital photodocumenter.

    Article Title: In Situ Cytokine Expression, Nitric Oxide and Reactive Oxygen Species Production During Primary Amoebic Meningoencephalitis Development.
    Article Snippet: β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-130656) was used as a loading control; it was dissolved in 5% nonfat dry milk in TBS-T and kept overnight at 4 ◦C. .. The membranes were incubated with anti-rabbit IgG labeled with HRP (Jackson ImmunoResearch, West Grove, PA, USA, 111–035–003; 1:5000), and the blots were revealed with a luminol kit reagent (Santa Cruz Biotechnology, Santa Cruz, CA, USA) using Kodak photographic film. ..

    Article Title: Lactylome Reprogramming Mediates Therapeutic Response and Adaptation to Neoadjuvant Chemotherapy in Esophageal Squamous Cell Carcinoma.
    Article Snippet: .. After three washes with TBST, membranes were incubated with HRP-conjugated secondary antibodies (111-035- 144/115-035-146, Jackson ImmunoResearch) for 1 h at room temperature. .. Following three additional washes with TBST, membranes were incubated with Enhanced chemiluminescence (ECL) substrate (MA0186, Meilunbio), and signals were visualized using a Tanon 4600 system.

    Labeling:

    Article Title: In Situ Cytokine Expression, Nitric Oxide and Reactive Oxygen Species Production During Primary Amoebic Meningoencephalitis Development.
    Article Snippet: β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-130656) was used as a loading control; it was dissolved in 5% nonfat dry milk in TBS-T and kept overnight at 4 ◦C. .. The membranes were incubated with anti-rabbit IgG labeled with HRP (Jackson ImmunoResearch, West Grove, PA, USA, 111–035–003; 1:5000), and the blots were revealed with a luminol kit reagent (Santa Cruz Biotechnology, Santa Cruz, CA, USA) using Kodak photographic film. ..



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    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) <t>HSP72,</t> HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.
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    Image Search Results


    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: Staining, Membrane

    HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: Staining, Membrane, Comparison

    HSP abundances in type I and II skeletal fibers from older adults pre- and post HIT exercise. Relative protein abundances of (A and B) HSP72, (C and D) HSP27, (E and F) pHSP27 Ser15, (G and H) αB-crystallin, and (I and J) pαB-crystallin Ser59 in fibers from old pre and old post HIT exercise. All fibers are expressed relative to the average old pre type I fibers or relative pre type II depending on fiber type. The same color is assigned to the same Individual, consistent in both graphs and all figures. * p < 0.05 and ** p < 0.01 indicated significant difference in paired t -test (except pHSP27 Ser15 Wilcoxon match-pair rank test). Representative blots are shown in . HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: HSP abundances in type I and II skeletal fibers from older adults pre- and post HIT exercise. Relative protein abundances of (A and B) HSP72, (C and D) HSP27, (E and F) pHSP27 Ser15, (G and H) αB-crystallin, and (I and J) pαB-crystallin Ser59 in fibers from old pre and old post HIT exercise. All fibers are expressed relative to the average old pre type I fibers or relative pre type II depending on fiber type. The same color is assigned to the same Individual, consistent in both graphs and all figures. * p < 0.05 and ** p < 0.01 indicated significant difference in paired t -test (except pHSP27 Ser15 Wilcoxon match-pair rank test). Representative blots are shown in . HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: