hrp conjugated goat anti rabbit igg secondary antibody (Jackson Immuno)
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Hrp Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 7244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp/Peroxidase+AffiniPure+Goat+Anti-Rabbit+IgG/pm42921007-125-25-35
Average 96 stars, based on 7244 article reviews
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Purification:Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an Immunohistochemistry:Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an Staining:Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration Article Snippet: All antibodies used for histology are listed in and were all diluted in PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology), except for the rabbit anti-pS6 (Ser240/244) antibody where PBS was replaced with TBS and the rabbit anti-apolipoprotein B (APOB), the goat anti-Apolipoprotein E (APOE), the rabbit anti-CFH, and goat anti-mouse complement C3 where Triton X-100 was replaced with 0.2% Saponin. .. Detection of signals used either fluorescent secondary antibodies (1:500, raised in donkey) that were purchased from Jackson Immuno Research and were purified F(ab)2 fragments that displayed minimal cross-reactivity with other species or an Western Blot:Article Title: TONSL/TONSOKU binding to histone H3.1 is required for DNA repair Article Snippet: The following primary antibodies were used for immunoblotting: anti-TONSL (NFKBIL2; MA5-17292, Thermo Fisher Scientific) and anti–α-tubulin (CP06, MilliporeSigma). .. Secondary antibodies used for immunoblotting were Goat anti-Rabbit immunoglobulin G (IgG), other:Article Title: Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development Article Snippet: Membranes blocked in 5% nonfat milk in Tris-buffered saline with Tween 20 for 1 hour at room temperature, followed by overnight incubation at 4°C with primary antibodies, including anti-HA (1:5000, Abcam, catalog no. Ab18181), anti-GFP (1:5000, Invitrogen, catalog no. 33-2600), anti-RFP (1:2000, ChromoTek, catalog no. pabr1), anti-FLAG (1:5000, Sigma-Aldrich, catalog no. F3165), and anti-ACTIN (1:10,000, Abcam, catalog no. Ab230169 ). Incubation:Article Title: Modifying VEGF-A mRNA by combinatorial optimization to enhance therapeutic efficacy for myocardial infarction. Article Snippet: After blocking, membranes were incubated overnight at 4°C with primary antibodies: rabbit anti-FLAG (1:1000, Cell Signaling Technology, #14793) and mouse antiGAPDH (1:5000, Proteintech, 60004-1-Ig). .. Membranes were washed five times with PBST (10 min each) and incubated with Article Title: DEAD-box RNA helicase DDX3X maintains the homeostasis of the Zika virus translation-replication cycle Article Snippet: The antibodies used were anti-β-actin (sc-477778, mouse, 1:5000, Santa Cruz Biotechnologies), anti-DDX3X (sc-365768, mouse, 1:3000, Abcam), anti-RIG-I (20566-1-AP, rabbit, 1:1000, Proteintech), DENV-2 anti-NS3 (Cat. 124252, rabbit, 1:3000, Genetex), ZIKV anti-NS3 and ZIKV anti-NS5 (rabbit, 1:3000, gently donated by Dr. Andres Merits, University of Tartu, Estonia). .. After incubation, the membranes were washed 3 times with TBS-T 1X and then incubated for 2 hours at room temperature with Article Title: In Situ Cytokine Expression, Nitric Oxide and Reactive Oxygen Species Production During Primary Amoebic Meningoencephalitis Development. Article Snippet: β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-130656) was used as a loading control; it was dissolved in 5% nonfat dry milk in TBS-T and kept overnight at 4 ◦C. .. The membranes were incubated with anti-rabbit IgG labeled with Article Title: Lactylome Reprogramming Mediates Therapeutic Response and Adaptation to Neoadjuvant Chemotherapy in Esophageal Squamous Cell Carcinoma. Article Snippet: .. After three washes with TBST, membranes were incubated with Labeling:Article Title: In Situ Cytokine Expression, Nitric Oxide and Reactive Oxygen Species Production During Primary Amoebic Meningoencephalitis Development. Article Snippet: β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-130656) was used as a loading control; it was dissolved in 5% nonfat dry milk in TBS-T and kept overnight at 4 ◦C. .. The membranes were incubated with anti-rabbit IgG labeled with |
